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(a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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rabbit anti human fibroblast activation protein alpha monoclonal primary antibody  (Danaher Inc)
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(a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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(a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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primary antibodies against hfap - by Bioz Stars, 2026-07
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chemokine array cultured primary lung fibroblasts  (DiaSorin Biotechnology)
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(a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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primary antibodies against er tr7  (Santa Cruz Biotechnology)
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(a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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mouse monoclonal anti-cd90 (thy-1)/fibroblast primary antibody  (Millipore)
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Millipore mouse monoclonal anti-cd90 (thy-1)/fibroblast primary antibody
Confocal laser scanning microscopy (CLSM) images showing actin filaments using phalloidin in control and treated fibroblasts marked with <t>CD90</t> (green), at different times of follow-up; magnification 63×.
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(a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

Journal: bioRxiv

Article Title: Bidirectional Crosstalk Between Bladder Cancer Cells and Normal Fibroblasts Drives Phenotypic Reprogramming and Modulates Chemosensitivity

doi: 10.1101/2025.09.09.675061

Figure Lengend Snippet: (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

Article Snippet: For NF, primary antibodies against fibroblast activation protein (FAP) and α-smooth muscle actin (αSMA) (Cell Signaling Technology) were applied at 1:100 overnight at 4°C.

Techniques: Flow Cytometry, Cell Culture, Activation Assay, Marker, Staining, Fluorescence, Co-Culture Assay, Expressing

Confocal laser scanning microscopy (CLSM) images showing actin filaments using phalloidin in control and treated fibroblasts marked with CD90 (green), at different times of follow-up; magnification 63×.

Journal: International Journal of Molecular Sciences

Article Title: Morphological and Biological Evaluations of Human Periodontal Ligament Fibroblasts in Contact with Different Bovine Bone Grafts Treated with Low-Temperature Deproteinisation Protocol

doi: 10.3390/ijms23095273

Figure Lengend Snippet: Confocal laser scanning microscopy (CLSM) images showing actin filaments using phalloidin in control and treated fibroblasts marked with CD90 (green), at different times of follow-up; magnification 63×.

Article Snippet: For double immunostaining, cells were incubated with mouse monoclonal anti-CD90 (Thy-1)/fibroblast primary antibody (Chemicon International Inc., Temecula, CA, USA), diluted 1:200 in blocking solution, O/N at 4 °C.

Techniques: Confocal Laser Scanning Microscopy